Blotting apparatus includes all components required for protein transfer from gel to membrane and subsequent membrane processing. Western blotting is a widely used technique by research and clinical labs to detect proteins with their specific antibody. Blots require an apparatus, or blotter to transfer the gel to membrane under wet, dry or semi-dry conditions. Transfer protocol depends on type of protein, gel thickness, and type of membrane. After transfer, proteins must be probed, stained, washed and detected. The assay can require crosslinking or hybridization to fix proteins, trays for washing, staining and destaining membranes, and holders to mount gels and blots. The process can be automated with equipment.