We provide PCR clean-up, agarose spin columns and minus EtBr spin columns for purification of single or double stranded PCR amplification fragments; as well as extraction of DNA fragments from Ethidium Bromide (EtBr) stained agarose gels.
Applications for these kits would include enzymatic reactions, automated sequencing, cloning, microarray analysis, ligation, restriction enzyme digestions, and PCR.
PCR Clean up
| Product no | Product description | Starting amount | Time required | Expected yield |
|---|---|---|---|---|
| 11732676001 (250 preps) 11732668001 (50 preps) |
High Pure PCR Product Purification Kit | 100 μl | <10 minutes for PCR product, and purify DNA from agarose gel slices in <20 minutes | |
| 4983912001 (200 preps) 4983955001 (50 preps) |
High Pure PCR Cleanup Micro Kit | Varies | 10 min | 85% recovers up to 20μg DNA |
| NA1020 (70 preps) | GenElute™ PCR Clean-Up Kit |
100 µL or 10 µg of DNA | <10 min | Up to 95% recovery |
| PCR9601 (1 x 96-well) PCR9604 (4 x 96-well) |
GenElute™ 96Well PCR Clean-up Kit | <100 µL PCR reaction | 45 min per plate | 75 – 95% recovery |
| D1947 (8RXN / 48RXN / 96RXN) |
Diffinity RapidTip® for PCR Purification | 25 µL | 1 min | Up to 95% recovery |
| D1947L (8RXN / 48RXN / 96RXN) |
Diffinity RapidTip® for PCR Purification, for Rainin LTS® | 25 µL | 1 min | Up to 95% recovery |
| D2947 (8RXN / 48RXN / 96RXN) |
Diffinity RapidTip®2 with polymerase removal | 50 µL | 1 min | Up to 95% recovery |
| D2947L (8RXN / 48RXN / 96RXN) |
Diffinity RapidTip®2 with polymerase removal, for Rainin LTS® | 50 µL | 1 min | Up to 95% recovery |
Gel Extraction
| Product no | Product description | Starting amount | Time required | Expected yield |
|---|---|---|---|---|
| 11696505001 | Agarose Gel DNA Extraction Kit | 100 mg gel slice | 60 min | Varies depending on size range of recovered DNA |
| NA1111 (70 preps) | GenElute™ Gel Extraction Kit | Up to 3.5 g gel slice | N/A | Up to 80% recovery |
| 56500 (70 preps) | GenElute™ Agarose Spin Columns | <200 mg gel slice | 10 min | Up to 80% recovery |
| 56501 (70 preps) | GenElute™ Minus EtBr Spin Columns | <200 mg gel slice | 10 min | Up to 70% recovery |