Reverse transcription polymerase chain reaction (RT-PCR) is a variation of standard PCR that involves the amplification of specific mRNA obtained from small samples. RT-PCR eliminates the need for the tedious mRNA purification process required for conventional cloning techniques. In RT-PCR, reverse transcriptase and an RNA sample are used in addition to the standard PCR reagents. The reaction mixture is heated to 37 ˚C, which enables the production of cDNA from the RNA sample by reverse transcription. This cDNA anneals to one of the primers leading to first-strand synthesis. Standard PCR proceeds and dsDNA is produced.
| Product no | Product Name | Features |
|---|---|---|
| HSRT100 | Enhanced Avian HS RT-PCR Kit | Flexible kit for one or two step RT-PCR
Highly purified eAMV-RT — transcribe difficult secondary structure at high temperature |
| 11939823001 | Titan One-Tube RT-PCR Kit | Reduces the error rate in PCR due to the proofreading capability
Threefold higher fidelity than standard Taq |
| 11855476001 | Titan One-Tube RT-PCR System | Reverse Transcriptase AMV for first strand cDNA synthesis
Expand High Fidelity Enzyme with proofreading for cDNA amplification |
| TOSRTRO | Transcriptor One-Step RT-PCR Kit | Uses hot-start one-step RT-PCR technology to ensure sensitive and robust reverse transcription.
Provides all the components required for one-step RT-PCR. |
| KK4752 | KAPA PROBE FAST One-Step | Designed for high-throughput, fast-cycling, one-step RNA quantification.
Suitable for use with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons). |
cDNA Synthesis Kits & Mixes
| Product no | Product Name | Features |
|---|---|---|
| STR1 | Enhanced Avian First-Strand Synthesis Kit | Highly purified eAMV-RT — transcribe difficult secondary structure at high temperature
Detects low abundance mRNA |
| GE27-9261-01 | First-strand cDNA Synthesis Kit | Pre-assembled bulk reaction mixes to save time and preven contamination
Includes two primers for mRNA with or without Poly(A) tails |
| 11483188001 | First Strand cDNA Synthesis Kit for RT-PCR | Includes AMV RT for higher thermostability vs. M-MuLV RT
Includes Rnase Inhibitor and dNTP Mix for PCR |
| RDRT | ReadyScript™ cDNA Synthesis Mix | Sensitive and easy-to-use solution for two-step RT-PCR
ReadyScript Enzyme is an RNAse H(+) modified M-MuLV RT |
| GE27-9263-01 | Ready-to-go™ T-Primed First-Strand Kit | 50 single-dose, ambient-temperature-stable, first-strand synthesis reactions
Contains Notl-(dT)18 Primer, Cloned FPLCpure M-MuLV RT, RNase Inhibitor, and dNTPs |
| 11117831001 | cDNA Synthesis Kit | Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA |
| THIFICDNARO | Transcriptor High Fidelity cDNA Synthesis Kit | Designed to reverse transcribe RNA with increased fidelity compared to other reverse transcriptases.
Optimized for two-step RT-PCR |
| 05893151001 | Transcriptor Universal cDNA Master | Convenient fast solution for cDNA synthesis and real-time PCR analysis. Whichever PCR instrument you are using, this master mix, supplied in two vials, realizes the full potential of your qRT-PCR assays. |
RT Enzymes — Reverse Transcriptases
| Product no | Product Name | Features |
|---|---|---|
| A4464 | Enhanced Avian Reverse Transcriptase | Greater sensitivity for low abundance mRNA
Best enzyme for transcribing through difficult secondary structure |
| ERT-RO | Expand™ Reverse Transcriptase | Transcribe cDNA fragments up to 13.5 kb
Can use cDNA or single-stranded DNA |
| M1302 | M-MLV Reverse Transcriptase | Uses single-stranded RNA, DNA or RNA-DNA hybrid
Generate first strand cDNA up to 7 kb |
| 10109118001 | Reverse Transcriptase AMV | Includes 5X First-strand cDNA Synthesis Buffer |
| 11062603001 | Reverse Transcriptase M-MuLV | Lacks endonuclease activity and has much lower RNase H activity vs AMV RT |
| TRANSRTRO | Transcriptor Reverse Trascriptase | Achieve high sensitivity in two-step RT-PCR. Transcriptor Reverse Transcriptase is used in conventional thermal cyclers and real-time PCR instruments (e.g., the LightCycler® Instruments).
Reverse transcribe difficult templates. The enzyme works well at elevated temperatures, thereby overcoming RNA secondary structure (e.g., GC-rich RNA templates) and facilitating optimal reaction conditions. |
Additional PCR Reagents
| Product no | Product Name | Features |
|---|---|---|
| 10814270001 | Primer for cDNA synthesis | Oligo-p(dt)15
Binds the 3′-poly-A tail of an mRNA to initiate synthesis of a cDNA |
| R7647 | Random Nonamers (cDNA Primers) | Random sequences of nine deoxyribonucleotide
May be used as universal, non-specific primers |
| RNAINH-RO | Protector RNase Inhibitor | Inhibit RNases during RNA isolation and purification
Active at pH 5-9 and at temperatures from 25 °C to 55 °C |
| R2520 | Ribonuclease Inhibitor - Human | Useful for in vitro inhibition of ribonucleases |
| 10786357001 | Ribonuclease H (RNase H) | Eliminate potential sources of PCR errors
Increase accessibility of primers during subsequent PCR |
| W4502 | Nuclease-Free Water | Molecular grade
DNase-free, RNase-free, Protease-free |