Routine PCR is used to produce an amplified amount of DNA for downstream applications, including clone length verification. Our products offer a variety of options to perform routine PCR. Our ReadyMix™ PCR reaction mixes and REDTaq® dye options provide an added convenience for your amplification reaction.
Taq DNA Polymerases
Taq DNA polymerase is a specialized thermostable enzyme isolated from Thermus aquaticus, a thermophillic bacterium. Taq is the most commonly used DNA polymerase for PCR and is now expressed recombinantly in E. coli. The enzyme is capable of 5′ → 3′ polymerase and exonuclease activity; SDS-PAGE examination finds no detectable contamination associated with endonucleases or exonucleases.
PCR Reaction Mixes
The ReadyMix™ PCR reaction mixes contain our high-quality Taq DNA polymerase, 99% pure dNTPs, and buffer in a 2X optimized reaction concentrate. This convenient product reduces pipetting and minimizes the risk of contamination by eliminating various mixing steps. Simply add template and primers to the ReadyMix™ Reaction Mix. PCR reaction mixes are formulated to address various PCR needs and can be purchased in combination with REDTaq® Dye for additional convenience.
| Product no | Product Name | Features |
|---|---|---|
| R2523 | REDTaq® ReadyMix PCR Reaction Mix | Includes Taq DNA polymerase, 99% pure deoxynucleotides, and reaction buffer in a 2× optimized reaction concentrate Includes REDTaq dye for identification |
| P4600 | ReadyMix™ Taq PCR Reaction Mix | Includes Taq DNA Polymerase, 99% pure deoxynucleotides and buffer in a 2× optimized reaction concentrate |
Roche AptaTaq Fast Start DNA Polymerase
| Product no | Product Name | Features |
|---|---|---|
| APTAT-RO | AptaTaq Fast DNA Polymerase | AptaTaq Fast products are ready-to-use reagents optimized for fast PCR on all thermal cyclers. Inbuilt aptamer mediated hot start assures high specificity. 4 minutes processing time only. Immediate enzyme activation. |
| ATPAM-RO | AptaTaq Fast PCR Master | AptaTaq Fast PCR Polymerase is a reversible, temperature-dependent hot start DNA amplification system with immediate activation. At temperatures above +60°C, AptaTaq Polymerase is activated, ideal for specific priming and fast PCR. |
Roche Expand™
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Roche FastStart™ Taq
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Hot start PCR Reagents
Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Hot Start PCR allows for reaction set up at room temperature without non-specific amplification and primer dimer formation. Whereas conventional PCR is often utilized to make exponential copies of your DNA target sequence without an additional temperature-sensitive reaction activation component.
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Custom DNA Oligos
| Standard DNA Oligos | Scale: 0.025, 0.05, 0.2, 1.0, 10, and 15 μmol Purification: Desalt, Cartridge, HPLC, and PAGE Sequence Length: Up to 120 bases |
| Easy Oligo | Amount: 3 OD Concentration: 100 µM Purification: Desalt Sequence Length: 15 - 30 bases |
| Pure & Simple Primers | Amount: 3 OD Purification: Cartridge Sequence Length: 12 - 35 bases Modifications: Not available Quality Control: 100% Mass Spectrometry Format: Supplied dry |
| iScale Oligos™ (mg & g quantity) | Larger quantities for in vivo, high throughput and commercial projects Quantity: 10, 25, 50, 100, 250, and 500 mg (Inquire for larger quantities) Purification: Desalt, RP-HPLC, & In vivo Sequence Length: 10 - 50 bases (Inquire for longer lengths) |
| Next-Gen Sequencing Oligos | Custom universal and index (barcode) adapter oligos for major sequencing platforms Suitable purity (percent full-length sequence) to maintain sequence read integrity Low levels of cross-contamination to facilitate proper sequence assembly |
| Commercial Oligos | Custom DNA Primers, qPCR Probes, and Next-Gen Sequencing Oligos for Life Science Research Tools, Molecular Diagnostics (ISO 13485 manufacturing available), and Laboratory Developed Tests |
| Locked Nucleic Acid | Available with Custom DNA Oligos (in all countries except for the United States) Increased thermal stability and hybridization specificity Easier and more specific designs for problematic target sequences |