SYBR Green based
KiCqStart SYBR® Green ReadyMix™ – Optimized for Bio-Rad, Qiagen, Instruments
SYBR® Green I is a commonly used fluorescent dye that binds double-stranded DNA molecules by intercalating between the DNA bases. It is used in quantitative PCR because the fluorescence can be measured at the end of each amplification cycle to determine, relatively or absolutely, how much DNA has been amplified.
| Product no | Product Name | Features |
|---|---|---|
| KCQS00 | KiCqStart SYBR Green qPCR ReadyMix | For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems |
| KCQS01 | KiCqStart SYBR Green qPCR ReadyMix Low ROX™ | For ABI and Stratagene instruments |
| KCQS02 | KiCqStart SYBR Green qPCR ReadyMix with ROX™ | For ABI instruments |
| KCQS03 | KiCqStart SYBR Green qPCR ReadyMix for iQ™ | With fluorescein for Bio-Rad systems |
JumpStart Taq ReadyMix for Conventional qPCR
| Product no | Product Name | Features |
|---|---|---|
| S9194 | SYBR Green JumpStart Taq ReadyMix™ for High Throughput QPCR | Prepared with internal reference dye for high-throughput qPCR |
| S4438 | SYBR Green JumpStart Taq ReadyMix for qPCR | Optimized with MgCl2 |
| S5193 | SYBR Green JumpStart Taq ReadyMix without MgCl2 | MgCl2 provided in package for optimization |
| S1816 | SYBR Green JumpStart Taq ReadyMix for qPCR, Capillary Formulation | Optimized for Roche LightCycler® |
Probe Based
Probe-based qPCR functions by recognition of a specific sequence on the desired PCR product. Unlike SYBR® Green qPCR methods, that use a intercalating dye to bind all double-stranded DNA, probe-based qPCR uses fluorescent-labeled target-specific probes. This technique yields increased specificity and sensitivity since only specific DNA molecules will be labeled. Other fluorescent dyes can also be combined with probe-based qPCR to label, identify, amplify, and quantify various sequences.
KiCqStart ReadyMix™ – Instrument Optimized
JumpStart Taq ReadyMix for Conventional qPCR
| Product no | Product Name | Features |
|---|---|---|
| D6442 | JumpStart Taq ReadyMix for High Throughput qPCR | Internal Reference Dye is provided for reaction normalization ROX dye is incorporated for ABI and other qPCR instruments |
| D7440 | JumpStart Taq ReadyMix for qPCR | Internal Reference Dye is provided for reaction normalization ROX dye is incorporated for ABI and other qPCR instruments |